The Berkeley Drosophila Genome Project (BDGP), a consortium of the Drosophila Genome Center, has produced over 240,000 ESTs derived from various tissues. The goal of the consortium includes full-insert sequencing and assembly of non-redundant cDNA clones. Annotated sequences of all Drosophila transcripts, including products of alternative splicing, will facilitate creation of a set of Drosophila Gateway clones for use in proteomics and other functional genomics projects.
A set of ~11,000 clones has been assembled from ~90,000 EST sequences, grouped or clotted, based on sequence identity from their 5' ends. One representative from each clot was rearrayed and its 5' and 3' ends sequenced, first to verify its identity, and second to recluster on the basis of the 3' sequence.
Generation of the Release 3 annotation of the genome made extensive use of our full-insert sequence data. In the course of that effort, human curators identified a total of 1,860 clones that have become the DGCr3. The DGCr3 currently includes clones chosen to replace clones with truncated ORFs, clones for genes that are not currently represented in the DGC, plus clones that represent putative alternative splicing forms.
For more information please see BDGP webpage (http://www.fruitfly.org/DGC/index.html). We also have 3 Databases for further information, DGC 1, DGC 2 and DGC 3.
This resource is available as bacterial glycerol stocks and can be ordered as individual clones, individual 384-well plates or complete set of clones in 39 plates (DGCr1.0 = 17 plates and DGCr2.0 = 16 plates & DGCr3.0 = 6).
Drosophilia - clone product information