Fugu BAC arrays

The Fugu rubripes BAC library, cloned into pBeloBACII (vector and sequence here), was constructed at Incyte using Fugu genomic DNA supplied by Dr Greg Elgar (School of Biological Sciences, Queen Mary, University of London, Mile End Road, London, E1 4NS ).

The library contains 42,624 clones arrayed in 111 (384-well) plates. The library plates are numbered from 176-286.

Over half (23,453) of these clones have been HindIII fingerprinted and have an average insert size of 80kb, extrapolating to a nine-fold coverage of the genome.  

This library - along with the cosmid library also available through Source BioScience LifeSciences - underpins and is being integrated into the Fugu genome draft assembly. The data will be linked together to form the long-range physical map of the Fugu genome.

All the data linking Fugu draft assembly to BAC and cosmid clones is available from the Fugu website, allowing selection of genomic clones from any given region of the Fugu genome for further investigation or sequence finishing.