The Xenopus Gene Collection (XGC) project is trans-NIH initiative, which was launched, as a subproject of the existing Mammalian Gene Collection. The general approach is to acquire or construct high-quality cDNA libraries. The libraries are screened by sequencing the 5' end of the clones. Putative full open reading frames clones are identified, and finally, selected clones are full-length sequence verified. The goal of the project is to make available a complete set of these full-length cDNA clones in Xenopus laevis and Xenopus tropicalis.
The XGC plan of work is:
The generation of libraries from different stages of development and adult tissues.
Sequencing of ~3,000 to 10,000 5' and 3' ESTs from each library.
All sequences are deposited into GenBank. The corresponding clones are available through the IMAGE clone distribution network and are fully accessible to the community.
Analysis of all available sequences into UniGene clusters.
Selection of candidate complete open reading frame (ORF) clones and their sequencing.
In summary a Xenopus Gene Collection (XGC) clone is identified by:
A unique IMAGE identifier [IMAGE ID] (cross-referring to one or more EST files in dbEST)
A unique MGC identifier [MGC_ID] (cross-referring to a sequence file in GenBank of the entire clone insert)
NB: In each of these subsets all clones on one plate share the same vector ( IRBH, IRBG and IRBN).
Details of the vectors used can be found here
Clones from the three subsets can be ordered as individual clones (streaks on agar) and 96-well plates (frozen bacterial stocks in glycerol).
Clones are ordered using the IMAGE ID on the standard I.M.A.G.E. order form (you cannot use the XGC_ID). If the clone ordered forms part of a XGC subset in the Source BioScience LifeSciences collection, you will receive the clone streaked out from IRBH, IRBG, or IRBN plates. Such clones will also have an XGC_ID which will be given on the email confirmation of your order. If a clone has an XGC_ID but is not yet part of the subset plates received by Source BioScience LifeSciences the clone will be streaked out from the main I.M.A.G.E. collection.