Xenopus tropicalis EST Clones


Please note that the clone(s) being sent have tested negative in our phage contamination assay. However these clones should still be handled with care as no phage assay can be guaranteed to be 100% accurate. 

What to do with your clones when you receive them

The clones have been streaked onto LB agar containing 50µg/ml ampicillin. Please store them at 4°C (not in a freezer). As soon as possible, re-streak the clones onto the same medium and incubate overnight at 37°C to obtain single colonies.

A selection of at least 10 single colonies should be picked and grown in LB broth containing ampicillin + 8% glycerol for subsequent freezing at -70°C. These frozen stocks can then be re-grown for plasmid isolation and purification.

Streaking out for single colonies is essential in case of contamination or cross-contamination, or if modifications of the original clone have taken place as the library has been replicated. If there is a problem, the more single colonies you streak out, the more likely it is that you can retrieve the original construct. You can also isolate the original clone from a contaminated sample by following this procedure.

 



Clone Labeling and Nomenclature

TEgg - clone from the X.tropicalis egg cDNA library.
The correct nomenclature of clone 35P4 from this library is TEgg035p04

TGas - clone from the X.tropicalis gastrula cDNA library.
The correct nomenclature of clone 87J8 from this library is TGas087j08

TNeu - clone from the X.tropicalis neurula cDNA library.
The correct nomenclature of clone 131B12 from this library is TNeu131b12

TTpa - clone from the X.tropicalis tadpole cDNA library.
The correct nomenclature of clone 140f12 from this library is TTpa140f12

THda - clone from the X.tropicalis tailbud head cDNA library.
The correct nomenclature of clone 150b06 from this library is THda150b06

TTba - clone from the X.tropicalis tailbud cDNA library.
The correct nomenclature of clone 155c07 from this library is TTba155c07

 

Vector

The vector used is pCS107 which was constructed in the Richard Harland's laboratory
(http://mcb.berkeley.edu/labs/harland/).


For vector map see http://mcb.berkeley.edu/labs/harland/media/pdfs/CS107.pdf


For polylinker details see http://mcb.berkeley.edu/labs/harland/media/pdfs/CS105,107polylinker.pdf

 

Subsequent publication

In subsequent publications or database entries the clone must be referred to by the full clone ID (e.g. TNeu128k06 is correct, 128k06 is incorrect). In addition, please acknowledge the originators of the clone and reference Gilchrist et al., 2004*, together with Source BioScience LifeSciences for supplying the clone.

 

*Gilchrist, M., Zorn, A. M., Voigt, J, Smith, J.C., Papalopulu, N. and Amaya, E. (2004). Defining a large set of full length clones from a Xenopus tropicalis EST project. Dev. Biol. 271: 498-516.