The CHORI-211 Zebrafish BAC library was generated by the BACPAC Resource Center (BPRC) at the Children’s Hospital Oakland Research Institute by Chung Li Shu and Kazutoyo Osoegawa in Pieter De Jong’s Laboratory. The library provides approximately 10x coverage of the Zebrafish genome.
The library was created from the Tübingen zebrafish strain male testis DNA, provided by Gerd-Joerg Rauch. The DNA was partially digested using a combination of EcoRI and EcoRI Methylase. The DNA was size selected and cloned into the pTARBAC2.1 vector, in E.coli DH10B.
There are 105,907 clones with an average insert size of around 165kb.
Use the NCBI Clone Finder tool, Zebrafish Ensembl or the ZFIN database to access the annotated zebrafish genome or go to the Sanger Institute for more information, and Zebrafish Vega for manually annotated zebrafish genome sequences.
Source BioScience offers the complete clone set of 288 384-well microtitre plates or individual clones, for research purposes only. We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.
Due to MTA requirements, CHORI-211 clones and libraries should be ordered online using GenomeCube, using the clone ID format e.g. CH211-15E22 to search for individual clones.
Please acknowledge the originators of the library, Robert Geisler and Pieter deJong, and Source BioScience for providing the clones in any publication arising out of using this resource.
Rauch, G.-J., Granato, M. and Haffter, P. (1997). A polymorphic zebrafish line for genetic mapping using SSLPs on high-percentage agarose gels. Technical Tips Online T01208.