Daniokey Zebrafish BAC clones

The Daniokey BAC library was created by the Ronald Plasterk Laboratory and Keygene N.V. The library provides approximately 10x coverage of the Zebrafish genome.

 The library was created from the Tübingen zebrafish strain male testis DNA, provided by R. Geisler. The DNA was partially digested with HindIII. The DNA was cloned into the pIndigoBAC-536 vector, in E.coli DH10B.

There are 104,064 clones with an average insert size of around 175kb. The Keygene library also contains ligations with extra large inserts of up to  230kb.

Use the NCBI Clone Finder tool,  Zebrafish Ensembl or the ZFIN database to access the annotated zebrafish genome or go to the Sanger Institute  for more information, and Zebrafish Vega  for manually annotated zebrafish genome sequences.

Format

Source BioScience offers  the complete clone set of 288 384-well microtitre plates or individual clones, for research purposes only. We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.

How to Order

Due to MTA requirements, Daniokey BAC clones and libraries should be ordered online using GenomeCube, using the clone ID format e.g. DKEY-252L8 or zk252L8 to search for individual clones.

Subsequent publication:

Please acknowledge the originators of the library, R.Plasterk and Keygene N.V., and Source BioScience for providing the clones in any publication arising out of using this resource.

References:

Suster, M.L., Abe, G., Schouw, A. and Kawakami, K. (2011). Transposon-mediated BAC transgenesis in zebrafish. Nat Protoc 6, 1998-2021

Howe, K, Clark, M.D. et al (2013) The zebrafish reference genome sequence and its relationship to the human genome Nature 496 (7446), 498-503