Protein Array Screening
General information
Source BioScience's Protein Arrays
contain the largest collection of arrayed proteins, which are
derived from several human tissues. Each Protein Array consists of
up to 27,648 E. coli-expressed proteins, which are printed in
duplicate on a PVDF membrane. Clones for the production of Protein
Arrays are sequenced and have undergone a stringent in-frame
analysis. Source BioScience's Protein Arrays are suitable for serum
screening and antibody epitope mapping.
Protein Array handbook
Clone resource flyer
Applications
- Serum screening: Arrayed proteins are
hybridised with (a minimum of 100 µl of customer-provided) human
patient sera, to find auto-antibodies against targets related to
disease, in e.g. autoimmune diseases.
- Antibody epitope mapping: Arrayed proteins are
incubated with monoclonal or purified antibodies (provided by
customer). Positive signals can be used to determine which epitope
is recognised by the antibody. This approach is valuable for all
clinicians and researchers who would like to work with antibodies
that are not fully characterised, especially for developing new
antibodies for diagnostics or treatments.
Screening
Patient serum (or plasma) or monoclonal or purified antibody,
respectively, is incubated with the desired Source BioSciences
Protein Array. Recognised proteins may be visualised with
an ECF-labelled secondary antibody. Customer may either
perform experiments on his or her own or employ Source BioScience
for scanning of proteins and analysis of results. Source BioScience
recommends to perform at least one replicate of the experiment.
Identified proteins and corresponding clones can be provided as
annotated clone lists, including EST sequence information, gene
symbol, EnsEMBL-gene ID, UniGene cluster ID, protein structure
information (if available), etc. Protein Array analysis images and
all quality-control results are electronically documented.
Fig.1: Serum Screening
using Source BioSciences Protein Arrays. The image shows approx.
150 signals (double spots). The position of these proteins is
determined and correlated with cDNA sequences encoding for the
proteins on the array.
Reference Customers
Dr. Derek
Murphey
Associate Director
Centre for Human Proteomics
Royal College of Surgeons in Ireland
123 St Stephen's Green
Dublin 2
Ireland
Tel.: +353 1 402 8518
Fax: +353 1 402 8514
Homepage
Dr. Mark T. Bedford
University of Texas
M.D. A. Cancer Center, Dept.of Carcinogenesis
Smithville, USA
Homepage
References Proteinarrays
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MCP (2005) 10.1074/mcp.M500198−MCP200
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