Protein Expression Services
Source BioScience offers a protein
expression service to advance and optimise research for customers.
We help you to express your protein of interest either in a
cellular or cell free system. With the availability of our
comprehensive clone collection, including Source BioScience
Full ORF (Open Reading Frame) Expression Clones, we adapt the
protein production to your demands. We offer you a selection of
different expression vectors with a variety of different tags and
protease cleavage sites. Based on your choice, we produce and
purify your protein, reaching optimal expression level and high
purity.
Figure 1: His6-tagged
recombinant fusion proteins, purified with IMAC chromatography
under native buffer conditions.
We also express the candidate proteins
identified by your protein array screen. This provides you
with the option to further characterise your candidate in
functional or biochemical assays.
Our customised Protein Expression Service offers:
Description of the bacterial expression
service
|
Module
|
Time
|
|
I. Cloning of the gene of interest into
a bacterial expression vector
- Subcloning the gene of interest into an appropriate bacterial
expression vector
- Verification of the expression clone by restriction enzyme
digest and sequencing
- Maxi-prep of the recombinant vector DNA
|
1.5 weeks
|
|
II. Expression screen
- Transformation of the expression clone into different bacterial
strains
- Mini-induction
- Solubility test and expression control with SDS-PAGE/Coomassie
stain, optionally also by Western Blotting
|
1 week
|
|
III. Mini-Expression
- Optimisation of expression conditions
- SDS-PAGE/Coomassie stain
|
1.5 weeks
|
|
IV. Large scale expression
- Protein expression up to 1000 ml culture volume
|
1 week
|
|
V. Protein purification
- Protein purification using His6-tag or GST-tag under native or
denaturing conditions
- Identification of the purified protein by Western Blot
- Dialysis of the protein
|
1 week
|
|
VI. Additional Services upon
request
|
|