CORF project - The Cloning of Open Reading Frames
The arrival of systems biology requires the cloning of large
sets of protein-encoding open reading frames (ORFs), to allow for
the functional studies of the resultant proteomes. The CORF has
generated and made available a set of clones covering the predicted
ORF′s for human chromosome 22.
Library specifications:
Ian Dunham′s team, at The Wellcome Trust Sanger Institute,
Hinxton, UK has constructed the set of clones in a pENTR vector.
The main advantage of using a recombinational cloning system is the
ease with which each ORF can be transferred from the Entry Vector
into any vector of choice by using the Gateway (Invitrogen) cloning
method.
Format:
Source BioScience LifeSciences offers the complete clone set of
6 96-well microtitre plates, or individual clones, for research
purposes only
The clones have been submitted to EMBL nucleotide sequence
database.
We can also make subsets of your chosen clones from the
collection. Please contact us
to discuss your requirements.
Reference:
A genome annotation-driven approach to cloning the human
ORFeome. Collins JE, Wright CL, Edwards CA, Davis MP,
Grinham JA, Cole CG, Goward ME, Aguado B, Mallya M, Mokrab Y,
Huckle EJ, Beare DM, Dunham I. Genome Biol. 2004;5(10):R84. Epub
2004 Sep 30.