Mutagenic Insertion and Chromosome Engineering Resource
(MICER)
Source BioScience LifeSciences are happy to announce the
distribution a second, fully indexed mouse resource, constructed in
Alan Bradley′s lab, The Wellcome Trust Sanger Institute, Hinxton,
UK.
Library specifications:
The library comprises of more than 93 thousand, constructed,
insertional targeting vectors from two different libraries MHPP
& MHPN. Approximately 6 thousand of these vectors can be used
directly to inactivate genes, with an average targeting efficiency
of 28%.
By using the combination of MHPP & MHPN vectors, 3′ Hprt and 5′
Hprt respectively, the disruption of both alleles of a gene may be
analysed. Furthermore, larger genomic changes may be engineered,
such as deletions, duplications translocations or inversions.
Each clone is a ready made targeting vector, incorporating a drug
resistance gene for drug targeting, the 5′ or 3′ portion of the
Hprt mini-gene, a loxP site for chromosome engineering. Finally, a
mouse coat-colour marker, for stock maintenance is a present on
each clone.
MICER clones are displayed on the Ensembl genome browser within a
DAS (Distributed Annotation Server). End sequenced vectors are
mapped to the C57BL/6J mouse genome assembly. 3′Hprt [MHPP] clones
are displayed as blue or green, whilst 5′Hprt [MHPN] clones are
displayed as black or red.
The MICER indexed library is a valuable tool that can facilitate
the rapid disruption of many genes, therefore providing a powerful
tool for accelerating genomic analysis.
If you would like further information regarding this resource,
please go to the following URL: www.sanger.ac.uk/PostGenomics/mousegenomics/
Format:
Source BioScience LifeSciences offers the complete clone set of
821 384-well microtitre plates, or individual clones, for research
purposes only.
Ensembl Mouse
We can also array subsets of your
chosen clones from the collection. Please contact
us to discuss your requirements.
Please note, the backbone vectors p5′ Hprt [MHPN] and p3′ Hprt
[MHPP] are also available at Source BioScience LifeSciences.
Reference:
Mutagenic insertion and chromosome engineering resource
(MICER). Adams DJ, Biggs PJ, Cox T, Davies R, van der
Weyden L, Jonkers J, Smith J, Plumb B, Taylor R, Nishijima I, Yu Y,
Rogers J, Bradley A. Nat Genet. 2004 Aug;36(8):867-71. Epub 2004
Jul 4. The Wellcome Trust Sanger Institute, Wellcome Trust Genome
Campus, Hinxton, Cambs, CB10 1SA, UK.