bMQ Mouse BAC vector

Genomic DNA was partially digested with Sau3A1 and cloned into BamHI linearized pBACe3.6

Clones are streaked onto LB agar containing chloramphenicol (20μg/ml). To use: re-streak the clone onto the same agar type, in such a way as to obtain single colonies. Incubate at 37°C overnight. Clones can be grown in LB broth + chloramphenicol, and glycerol added after growth to a concentration of 8% for long-term storage at -70°C.

1 gatccgcgga attcgagctc acgcgtactg atgcatgatc cgggtttaaa cccagtactc
61 tagatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt
121 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
181 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
241 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
301 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
1021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
1081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
1141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
1201 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
1261 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
1321 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
1381 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
1441 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
1501 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
1561 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
1621 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
1681 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
1741 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
1801 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
1861 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
1921 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
1981 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
2041 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
2101 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
2161 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
2221 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
2281 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
2341 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
2401 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
2461 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
2521 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
2581 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
2641 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
2701 cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgggga tctagagtac
2761 tggttgaaac ccggatcatg catcagtacg cgtgagctcg aattccgcgg atccttctat
2821 agtgtcacct aaatgtcgac ggccaggcgg ccgccaggcc tacccactag tcaattcggg
2881 aggatcgaaa cggcagatcg caaaaaacag tacatacaga aggagacatg aacatgaaca
2941 tcaaaaaaat tgtaaaacaa gccacagttc tgacttttac gactgcactt ctggcaggag
3001 gagcgactca agccttcgcg aaagaaaata accaaaaagc atacaaagaa acgtacggcg
3061 tctctcatat tacacgccat gatatgctgc agatccctaa acagcagcaa aacgaaaaat
3121 accaagtgcc tcaattcgat caatcaacga ttaaaaatat tgagtctgca aaaggacttg
3181 atgtgtggga cagctggccg ctgcaaaacg ctgacggaac agtagctgaa tacaacggct
3241 atcacgttgt gtttgctctt gcgggaagcc cgaaagacgc tgatgacaca tcaatctaca
3301 tgttttatca aaaggtcggc gacaactcaa tcgacagctg gaaaaacgcg ggccgtgtct
3361 ttaaagacag cgataagttc gacgccaacg atccgatcct gaaagatcag acgcaagaat
3421 ggtccggttc tgcaaccttt acatctgacg gaaaaatccg tttattctac actgactatt
3481 ccggtaaaca ttacggcaaa caaagcctga caacagcgca ggtaaatgtg tcaaaatctg
3541 atgacacact caaaatcaac ggagtggaag atcacaaaac gatttttgac ggagacggaa
3601 aaacatatca gaacgttcag cagtttatcg atgaaggcaa ttatacatcc ggcgacaacc
3661 atacgctgag agaccctcac tacgttgaag acaaaggcca taaatacctt gtattcgaag
3721 ccaacacggg aacagaaaac ggataccaag gcgaagaatc tttatttaac aaagcgtact
3781 acggcggcgg cacgaacttc ttccgtaaag aaagccagaa gcttcagcag agcgctaaaa
3841 aacgcgatgc tgagttagcg aacggcgccc tcggtatcat agagttaaat aatgattaca
3901 cattgaaaaa agtaatgaag ccgctgatca cttcaaacac ggtaactgat gaaatcgagc
3961 gcgcgaatgt tttcaaaatg aacggcaaat ggtacttgtt cactgattca cgcggttcaa
4021 aaatgacgat cgatggtatt aactcaaacg atatttacat gcttggttat gtatcaaact
4081 ctttaaccgg cccttacaag ccgctgaaca aaacagggct tgtgctgcaa atgggtcttg
4141 atccaaacga tgtgacattc acttactctc acttcgcagt gccgcaagcc aaaggcaaca
4201 atgtggttat cacaagctac atgacaaaca gaggcttctt cgaggataaa aaggcaacat
4261 ttgcgccaag cttcttaatg aacatcaaag gcaataaaac atccgttgtc aaaaacagca
4321 tcctggagca aggacagctg acagtcaact aataacagca aaaagaaaat gccgatactt
4381 cattggcatt ttcttttatt tctcaacaag atggtgaatt gactagtggg tagatccaca
4441 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca
4501 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt
4561 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga
4621 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
4681 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
4741 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
4801 aaacatgaga attgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag
4861 ttattaggtc cctcgactat agggtcaccg tcgacagcga cacacttgca tcggatgcag
4921 cccggttaac gtgccggcac ggcctgggta accaggtatt ttgtccacat aaccgtgcgc
4981 aaaatgttgt ggataagcag gacacagcag caatccacag caggcataca accgcacacc
5041 gaggttactc cgttctacag gttacgacga catgtcaata cttgcccttg acaggcattg
5101 atggaatcgt agtctcacgc tgatagtctg atcgacaata caagtgggac cgtggtccca
5161 gaccgataat cagaccgaca acacgagtgg gatcgtggtc ccagactaat aatcagaccg
5221 acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc
5281 gtggttccag actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata
5341 atcagaccga cgatacgagt gggaccatgg tcccagacta ataatcagac cgacgatacg
5401 agtgggaccg tggtcccagt ctgattatca gaccgacgat acgagtggga ccgtggtccc
5461 agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagactaa taatcagacc
5521 gacgatacga gtgggaccgt ggtcccagtc tgattatcag accgacgata caagtggaac
5581 agtgggccca gagagaatat tcaggccagt tatgctttct ggcctgtaac aaaggacatt
5641 aagtaaagac agataaacgt agactaaaac gtggtcgcat cagggtgctg gcttttcaag
5701 ttccttaaga atggcctcaa ttttctctat acactcagtt ggaacacgag acctgtccag
5761 gttaagcacc attttatcgc ccttatacaa tactgtcgct ccaggagcaa actgatgtcg
5821 tgagcttaaa ctagttcttg atgcagatga cgttttaagc acagaagtta aaagagtgat
5881 aacttcttca gcttcaaata tcaccccagc ttttttctgc tcatgaaggt tagatgcctg
5941 ctgcttaagt aattcctctt tatctgtaaa ggctttttga agtgcatcac ctgaccgggc
6001 agatagttca ccggggtgag aaaaaagagc aacaactgat ttaggcaatt tggcggtgtt
6061 gatacagcgg gtaataatct tacgtgaaat attttccgca tcagccagcg cagaaatatt
6121 tccagcaaat tcattctgca atcggcttgc ataacgctga ccacgttcat aagcacttgt
6181 tgggcgataa tcgttaccca atctggataa tgcagccatc tgctcatcat ccagctcgcc
6241 aaccagaaca cgataatcac tttcggtaag tgcagcagct ttacgacggc gactcccatc
6301 ggcaatttct atgacaccag atactcttcg accgaacgcc ggtgtctgtt gaccagtcag
6361 tagaaaagaa gggatgagat catccagtgc gtcctcagta agcagctcct ggtcacgttc
6421 attacctgac catacccgag aggtcttctc aacactatca ccccggagca cttcaagagt
6481 aaacttcaca tcccgaccac atacaggcaa agtaatggca ttaccgcgag ccattactcc
6541 tacgcgcgca attaacgaat ccaccatcgg ggcagctggt gtcgataacg aagtatcttc
6601 aaccggttga gtattgagcg tatgttttgg aataacaggc gcacgcttca ttatctaatc
6661 tcccagcgtg gtttaatcag acgatcgaaa atttcattgc agacaggttc ccaaatagaa
6721 agagcatttc tccaggcacc agttgaagag cgttgatcaa tggcctgttc aaaaacagtt
6781 ctcatccgga tctgaccttt accaacttca tccgtttcac gtacaacatt ttttagaacc
6841 atgcttcccc aggcatcccg aatttgctcc tccatccacg gggactgaga gccattacta
6901 ttgctgtatt tggtaagcaa aatacgtaca tcaggctcga accctttaag atcaacgttc
6961 ttgagcagat cacgaagcat atcgaaaaac tgcagtgcgg aggtgtagtc aaacaactca
7021 gcaggcgtgg gaacaatcag cacatcagca gcacatacga cattaatcgt gccgataccc
7081 aggttaggcg cgctgtcaat aactatgaca tcatagtcat gagcaacagt ttcaatggcc
7141 agtcggagca tcaggtgtgg atcggtgggc agtttacctt catcaaattt gcccattaac
7201 tcagtttcaa tacggtgcag agccagacag gaaggaataa tgtcaagccc cggccagcaa
7261 gtgggcttta ttgcataagt gacatcgtcc ttttccccaa gatagaaagg caggagagtg
7321 tcttctgcat gaatatgaag atctggtacc catccgtgat acattgaggc tgttccctgg
7381 gggtcgttac cttccacgag caaaacacgt agccccttca gagccagatc ctgagcaaga
7441 tgaacagaaa ctgaggtttt gtaaacgcca cctttatggg cagcaacccc gatcaccggt
7501 ggaaatacgt cttcagcacg tcgcaatcgc gtaccaaaca catcacgcat atgattaatt
7561 tgttcaattg tataaccaac acgttgctca acccgtcctc gaatttccat atccgggtgc
7621 ggtagtcgcc ctgctttctc ggcatctctg atagcctgag aagaaacccc aactaaatcc
7681 gctgcttcac ctattctcca gcgccgggtt attttcctcg cttccgggct gtcatcatta
7741 aactgtgcaa tggcgatagc cttcgtcatt tcatgaccag cgtttatgca ctggttaagt
7801 gtttccatga gtttcattct gaacatcctt taatcattgc tttgcgtttt tttattaaat
7861 cttgcaattt actgcaaagc aacaacaaaa tcgcaaagtc atcaaaaaac cgcaaagttg
7921 tttaaaataa gagcaacact acaaaaggag ataagaagag cacatacctc agtcacttat
7981 tatcactagc gctcgccgca gccgtgtaac cgagcatagc gagcgaactg gcgaggaagc
8041 aaagaagaac tgttctgtca gatagctctt acgctcagcg caagaagaaa tatccaccgt
8101 gggaaaaact ccaggtagag gtacacacgc ggatagccaa ttcagagtaa taaactgtga
8161 taatcaaccc tcatcaatga tgacgaacta acccccgata tcaggtcaca tgacgaaggg
8221 aaagagaagg aaatcaactg tgacaaactg ccctcaaatt tggcttcctt aaaaattaca
8281 gttcaaaaag tatgagaaaa tccatgcagg ctgaaggaaa cagcaaaact gtgacaaatt
8341 accctcagta ggtcagaaca aatgtgacga accaccctca aatctgtgac agataaccct
8401 cagactatcc tgtcgtcatg gaagtgatat cgcggaagga aaatacgata tgagtcgtct
8461 ggcggccttt ctttttctca atgtatgaga ggcgcattgg agttctgctg ttgatctcat
8521 taacacagac ctgcaggaag cggcggcgga agtcaggcat acgctggtaa ctttgaggca
8581 gctggtaacg ctctatgatc cagtcgattt tcagagagac gatgcctgag ccatccggct
8641 tacgatactg acacagggat tcgtataaac gcatggcata cggattggtg atttcttttg
8701 tttcactaag ccgaaactgc gtaaaccggt tctgtaaccc gataaagaag ggaatgagat
8761 atgggttgat atgtacactg taaagccctc tggatggact gtgcgcacgt ttgataaacc
8821 aaggaaaaga ttcatagcct ttttcatcgc cggcatcctc ttcagggcga taaaaaacca
8881 cttccttccc cgcgaaactc ttcaatgcct gccgtatatc cttactggct tccgcagagg
8941 tcaatccgaa tatttcagca tatttagcaa catggatctc gcagataccg tcatgttcct
9001 gtagggtgcc atcagatttt ctgatctggt caacgaacag atacagcata cgtttttgat
9061 cccgggagag actatatgcc gcctcagtga ggtcgtttga ctggacgatt cgcgggctat
9121 ttttacgttt cttgtgattg ataaccgctg tttccgccat gacagatcca tgtgaagtgt
9181 gacaagtttt tagattgtca cactaaataa aaaagagtca ataagcaggg ataactttgt
9241 gaaaaaacag cttcttctga gggcaatttg tcacagggtt aagggcaatt tgtcacagac
9301 aggactgtca tttgagggtg atttgtcaca ctgaaagggc aatttgtcac aacaccttct
9361 ctagaaccag catggataaa ggcctacaag gcgctctaaa aaagaagatc taaaaactat
9421 aaaaaaaata attataaaaa tatccccgtg gataagtgga taaccccaag ggaagttttt
9481 tcaggcatcg tgtgtaagca gaatatataa gtgctgttcc ctggtgcttc ctcgctcact
9541 cgagggcttc gccctgtcgc tcaactgcgg cgagcactac tggctgtaaa aggacagacc
9601 acatcatggt tctgtgttca ttaggttgtt ctgtccattg ctgacataat ccgctccact
9661 tcaacgtaac accgcacgaa gatttctatt gttcctgaag gcatattcaa atcgttttcg
9721 ttaccgcttg caggcatcat gacagaacac tacttcctat aaacgctaca caggctcctg
9781 agattaataa tgcggatctc tacgataatg ggagattttc ccgactgttt cgttcgcttc
9841 tcagtggata acagccagct tctctgttta acagacaaaa acagcatatc cactcagttc
9901 cacatttcca tataaaggcc aaggcattta ttctcaggat aattgtttca gcatcgcaac
9961 cgcatcagac tccggcatcg caaactgcac ccggtgccgg gcagccacat ccagcgcaaa
10021 aaccttcgtg tagacttccg ttgaactgat ggacttatgt cccatcaggc tttgcagaac
10081 tttcagcggt ataccggcat acagcatgtg catcgcatag gaatggcgga acgtatgtgg
10141 tgtgaccgga acagagaacg tcacaccgtc agcagcagcg gcggcaaccg cctccccaat
10201 ccaggtcctg accgttctgt ccgtcacttc ccagatccgc gctttctctg tccttcctgt
10261 gcgacggtta cgccgctcca tgagcttatc gcgaataaat acctgtgacg gaagatcact
10321 tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt
10381 ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
10441 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
10501 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
10561 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
10621 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
10681 acattcttgc ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg
10741 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
10801 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
10861 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
10921 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
10981 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
11041 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
11101 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
11161 ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata
11221 ataagcggat gaatggcaga aattcgatga taagctgtca aacatgagaa ttggtcgacg
11281 gcgcgccaaa gcttgcatgc ctgcagccgc gtaacctggc aaaatcggtt acggttgagt
11341 aataaatgga tgccctgcgt aagcggggca catttcatta cctctttctc cgcacccgac
11401 atagataata acttcgtata gtatacatta tacgaagtta tctagtagac ttaattaagg
11461 atcgatccgg cgcgccaata gtcatgcccc gcgcccaccg gaaggagctg actgggttga
11521 aggctctcaa gggcatcggt cgagcttgac attgtaggac tatattgctc taataaattt
11581 gcggccgcta atacgactca ctatagggag ag