Synthetic genes
Why synthetic genes?
Synthetic genes are open reading frame sequences from
publicly annotated genes generated synthetically. A synthetic gene
is the product of choice, if no natural clone is available or if
available clones do not fulfil your specific requirements, such as:
100% sequence identity to a database sequence, DNA sequence
optimisation for maximisation of protein yield in your
preferred expression system, e.g. E.coli or yeast.
Search and order
Synthetic genes can be readily identified and ordered via Source
BioScience's intuitive-to-use online search tool GenomeCube®.
Alternatively, please contact the Source BioScience sales team.
You may send us various Accession Ids corresponding to nucleotide
sequences (e.g. GenBank, Ensembl) as well as protein Ids (e.g.
UniProt). Usually they will be delivered within 4-6 weeks depending
on the length of the synthetic construct.
Application
Synthetic genes are of primary interest for
- Genes for which no clones are available or hard to obtain
otherwise (e.g. Full ORF clones, splice variants)
- Increased expression yields due to the sequence
optimisation
- Design of the sequence due to your specific requirements (e.g.
introduction of point mutations, insertions or deletions, epitope
and domain shuffling, introduction of fusion tags and particular
restriction sites)
Product range
- Protein expression system specific sequence optimisation
in order to maximise protein yield
- Gene synthesis
- Generation of gene variants or randomised libraries for
structural or functional analyses
- Cloning of synthetic gene into the vector of your choice
- Comprehensive quality control including full-length
sequencing
- Protein expression, purification and characterisation as a
separate service. More
As a technology/source independent provider of synthetic genes,
Source BioScience is able to support your survey for the most
economic gene synthesis approach that fits your scientific
requirements best. In order to do so, we offer you an in-depth
analysis of your requirements in the course of a counseling
interview. As a result we provide you with a detailed offer
reflecting your needs and the best fitting and most economic
approach yet available on the international market. Contact
Disclaimer
Source BioScience reserves the right to withdraw from a
gene synthesis order if the encoding sequence comprises hazardous,
complex or genetically instable sequence elements.