WGA DNA
Whole Genome Amplified (WGA) DNA aliquots have been prepared
from a variety of organisms such as Human, Mouse, Rat, Chicken,
Dog, Bovine, Fugu and S. cerevisiae. WGA aliquots have
been prepared to the highest quality. The researcher can utilise
the aliquots in a variety of applications such as PCR, library
construction, southern blotting and SNP analysis.
- Random primers, which are exonulease resistant, anneal to a
single strand of genomic DNA template.
- Phi 29 polymerase moves along the DNA template strand. The DNA
polymerase has a 3´ -> 5´ exonuclease proof reading activity
that maintains the high fidelity during replication.
- Phi 29 displaces the complementary strand.
- The displaced strand becomes a template for the subsequent
rounds of replication resulting in elevated yields of high
molecular weight DNA.
WGA DNA Aliquots
Supplied: 50µg of DNA 1µg/µl in sterile distilled water.
Store at 4°C
Click on the link below to order
Fugu rubripes genomic
DNA
Total Fugu genomic DNA was prepared by proteinase K digestion of
testis followed by phenol/chloroform extraction and extensive
dialysis against 10mM Tris-HCl pH 7.4, 1mM EDTA. We supply aliquots
containing 20µg total genomic DNA/aliquot.
Supplied: 20µl at 1 µg/ µl in TE buffer Store at 4°C
Click on the link below to order
Source BioScience LifeSciences also offers a
comprehensive WGA service that facilitates the rescue of
minute quantities of DNA such as buccal swabs, mouthwashes and
finger pricks. Furthermore, if you would like to reduce
time-consuming genomic preparations, whether eukaryotic or
prokaryotic, why not try amplifying your precious last microlitre
by using WGA. For further information please visit our Whole Genomic Amplification
Service