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Library Types

RNAi/miRNA clones

RNA interference (RNAi) is a process of post-transcriptional gene silencing by which double stranded RNA (dsRNA), when introduced into a cell, causes sequence-specific degradation of homogolous mRNA sequences.
Micro RNA is an endogenous, single-stranded RNA, typically 21-23 base pairs long, that is thought to be involved in gene regulation (especially inhibition of protein expression).

Genomic clones

Genomic clones are large (40-100kbp) sections of DNA typically harboured in fosmid, cosmid or BAC vectors. All resources have been end, or fully sequenced.

ORF clones

Open Reading Frame (ORF) clones contain the entire coding sequences for genes. Vectors can be used for either direct expression or recombined into an expression construct, depending on which library is chosen.

cDNA/EST clones

Expressed Sequence Tag, is a segment of a cDNA clone that has been sequenced. EST Clones are cDNA clones that have been sequenced once (single pass, non-edited), from one or both ends of the DNA insert, using universal primers which are complementary to the vector and close to the multiple cloning site.

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