Whole Genome Amplificaiton (WGA)
What method do you use for whole genome
amplification?
We use the Multiple Displacement Amplification (MDA) method. MDA
produces amplified DNA with comprehensive coverage of all gene
sequences and unprecedented low bias. This revolutionary method
allows researchers to rescue minute quantities of DNA and gives
them greater flexibility to work with smaller samples such as
buccal swabs, mouthwashes and finger pricks. In addition, a quality
assessment on the amplified DNA is performed to effectively predict
the usability of amplified DNA for downstream uses such as microsatellite genotyping, Taqman assays,
10K, 100K and 500K SNP microarray assays all of which are
provided by Source BioScience LifeSciences.
Can you give me more details on WGA?
Our specifications include the following:
- PicoGreen quantitation assay on the samples prior to
amplification when the samples provided are genomic
DNA
- Either, Standard: WGA in reaction volume of 100 ul and
an expected yield of approximately 100 ug of amplified DNA, or,
Customised: WGA with customised yield is possible.
- Standard quality assessment of the amplified human DNA
samples, i) DNA quantitation of the WGA yield by the PicoGreen
assay and ii) Loci representation QC by TaqMan assay on two loci
(1004 and 699). Based on these values, Source BioScience
LifeSciences will provide a usability score for each sample that
will permit the customer to decide which samples will need to be
eliminated in the ensuing study
- Delivery of the amplified product and a
report
How should I send my DNA samples to you?
The DNA samples to be amplified should be shipped in 96 well PCR
plates. Each plate should have a maximum of 84 DNA samples
leaving all of row H empty. The DNA samples should
be in TE (10 mM Tris-HCl pH 7.5, 1 mM EDTA), however samples in 10
mM Tris-HCl (pH 7.5) or water will also be accepted. The DNA
concentration should be more than 5 ng/μl in a total volume of
20ul. The plates should be tightly sealed with strip caps and the
samples frozen before shipment. Wrap each plate in some type of
padded material such as foam wrap or bubble wrap before packaging
on dry ice. You must pay due regard to the postal regulations that
are in force at the time of sending.
How should I send other samples types to you for whole
genome amplification?
Blood, buffy coat, cell culture and blood spots to be amplified
should be shipped in 96 well PCR plates. Each plate should have a
maximum of 84 samples leaving all of row H empty.
The total volume of the samples should be 5-50 μl with a minimum of
1000 cells/5μl. The plates should be tightly sealed with strip caps
and the samples frozen before shipment. Wrap each plate and
dispatch as above.
I still have some questions, who can I speak
to?
Please contact us for further information. Our
specialist will be more than happy to discuss your requirements in
greater details and can provide you with a custom proposal of work
with pricing specific to your project.