Real Time PCR


Real time PCR is a powerful tool to analyse DNA and RNA quantitatively. In contrast to traditional PCR end-point detection, real time PCR collects data as the reaction is proceeding. By measuring the amounts of PCR product in the exponential phase of the reaction, real time PCR provides fast, precise and sensitive way for DNA and RNA quantitation.

 

Application

 

  • Validation of expression of genes resulting from microarray studies
  • Quantitation of gene expression
  • Detection and quantitation of DNA
  • Pre-designed and Custom assays (including design) available

 

 

Platform

 

  • Gold standard TaqMan® assay for robust high throughput applications.
  • High throughput liquid handling instrumentation
  • ABI PRISM 7900HT Sequence Detection Systems permit high throughput data collection

 

Source BioScience LifeSciences will...

 

  • Assist you in the design of the assays;
  • QC your total RNA and convert it into cDNA and
  • Run and analyse your samples returning your data on CD-R or via a secure web server.

 

 

For more information please contact:

Email: Gene Expression Team