The Applied Biosystems Low Density Array (microfluidic card) screens multiple samples against custom-selected gene panels, providing a cost-effective solution to validating the expression of large numbers of genes using smaller numbers of samples. Up to 380 genes can be rapidly examined on the same array using the well-established ABI TaqMan® gene expression reagents. These are predesigned real-time PCR assays available for human, mouse, and rat gene expression research. The TaqMan® Assay can be customised to a design of 6 configurations: from 11 genes with 4 replicates run on 8 samples, to 380 genes with no replicates run on a single sample. The Low Density array minimizes reagent consumption, as reaction volumes of ~2ul not only reduce the cost in terms of consumables but also limit the amount of RNA required per experiment.
Real-time PCR expression studies using Applied Biosystems Low Density Array (microfluidic card) can be used for: