Applied Biosystems TaqMan ® SNP Genotyping
Description
Single Nucleotide Polymorphisms are bi-allelic, single base-pair
changes between the DNA sequences of an individual. They can exist
within a population at any frequency although those with a
frequency above 20% are generally most useful in genetic mapping
studies. SNPs are found throughout the genome averaging one SNP
every 500 to 1000bp. They can affect translation and transcription.
In some circumstances they lead to profound disease whereas
elsewhere will have no obvious effect. SNPs have also been used in
pharmacogenomic experiments to predict why some individuals respond
better to certain therapeutics.
Application
The special virtues of SNPs for genetic analysis are their ease
of discovery and assay, and their ease of analysis. Their high
density in the human genome and their effects, in some cases, on
transcription and translation make SNPs valuable for association
studies.
The TaqMan ® platform is suitable for projects of 10s of SNPs
and 1000s of Samples. For higher throughput projects customers
should consider SNPlex
Platforms
- TaqMan assay for medium throughput applications.
- MATRIX PlateMatePlus for high throughput liquid handling.
- KBiosystems Super Duncan thermal cycler.
- ABI PRISM 7900HT Sequence Detection Systems permit high
throughput data collection.
Why use Source BioScience LifeSciences?
- Quality: Rapid turnaround and accurate
results. All data is sent in strictest confidence to you on CD-ROM,
e-mail or web server.
- Pricing: Our high throughput reduces unit
labour and capital costs meaning we can offer exceptional value for
money. Furthermore, savings are made by bulk purchase of
reagents.
- Personal Touch: We can also design, validate
and genotype user-defined SNPs.
- Commitment to improvement: We are continuously
introducing new technologies to reduce costs, shorten timelines and
reduce consumables including amounts of your DNA.
Contact
E-mail: Genotyping
Team