Microsatellite Genotyping
Description
Microsatellites are repetitive sequence elements - usually a
dinucleotide such as (CA)n. There are tens of thousands of
microsatellites in the human genome. Any one microsatellite locus
will have a number of different alleles, each with a different
number of repeat units.
We have earned a strong reputation in microsatellite typing. Our
present operations evolved from the highly regarded 'HGMP Linkage
Hotel', and we now offer high throughput contract research as well
as data analysis.
Application
Microsatellite typing has been used in thousands of linkage
analyses of Mendelian traits to detect co-segregation of a distinct
phenotype with a particular microsatellite allele in a family. It
has also been used to identify genes in complex diseases.
- Source BioScience LifeSciences has optimised protocols for
using ABI PRISM® Linkage Mapping Sets. These are sets of
microsatellite markers distributed at medium density (10
centiMorgan) and high density (5cM) across the human genome.
- Source BioScience LifeSciences also designs, validates and
applies user-defined microsatellites.
Platforms
- MATRIX Platemate Plus for high throughput liquid handling.
- ABI Prism 3730 and 3100 Genetic Analysers for high throughput
PCR product analysis
- Automated data analysis
Why use Source BioScience LifeSciences?
- Quality: Backed by nearly ten years experience
and with a track record of successes in detecting linkage, our
contract research is fast and accurate.
- Pricing: Our high throughputs reduce unit
labour and capital costs meaning that we can offer exceptional
value for money. Furthermore, savings are made by bulk purchase of
reagents.
- Personal Touch: We work with each customer to
ensure optimisation of experimental design. All data is sent in
strictest confidence to you on CD-ROM (or e-mail if
preferred).
- Commitment to improvement: We are continuously
introducing new technologies to reduce costs, shorten timelines and
reduce consumables including your DNA.
Publications
Click here for
publications
Contact
E-mail: Genotyping
Team