SNP genotyping using Illumina BeadArray
and Assay technologies
Description
As a leading provider of genomic research service in the UK,
Source BioScience LifeSciences has recently added the Illumina
BeadArray technology to the range of platform available for SNP
genotyping.
BeadArray Technology
In the heart of the BeadArray technology is the use of 3μM
beads that randomly assemble in either a fibre optic bundle
substrate or a silica slide substrate. These two substrates form
the basis of the two different array formats available: the
96-sample array matrix format based on fibre optic bundle and the
BeadChip format based on the silica slide. Each bead contains a
large number of a specific oligonucleotide and a 23 base address
code. The randomly assembled beads are then decoded through a
series of sequential hybridisation processes.
Assay Technology
Illumina assay technologies for SNP genotyping include the
GoldenGate assay and the Infinium II assay.
- The GoldenGate assay is used to interrogate 384 - 1536 SNPs
simultaneously by using allele specific extension and ligation. The
high level of multiplexing minimises the time and reagents required
for processing. Researchers can select SNP loci of choice to form
customer panels for targeted genotyping or an expanding selection
of standard panels is also available
- The Infinium II assay, coupled with BeadChips, allows large
scale interrogation of variations in the human genome at unlimited
levels of loci multiplexing. Whole genome BeadChips covering SNP
content ranging from 240,000 to 1 million and iSelect Infinium
Custom Genotyping Panels are available through Source BioScience
LifeSciences. The SNPs contained on the whole genome BeadChip range
of products are derived from Phase I HapMap data, and contain
mainly tag SNPs. The use of tag SNPs greatly improves the power of
association studies.
Genotyping options
Applications
- Whole-genome association studies
- Fine-mapping studies
- Whole genome LOH / copy number variation analysis
- Disease-associated copy number variation analysis
Highlights
- Each bead type is represented more than 20 times on the
substrate. This strategy provides the statistical accuracy of
multiple measurements and results in providing high quality, highly
reproducible data.
- The decoding process means that each bead has gone through a
functional validation process, allowing Quality Control of every
feature of every array to ensure that each array element is present
and functional.
- New bead pools are easily synthesised, allowing fast turnaround
for custom panels
- Powerful assay technologies allow high levels of multiplexing,
allowing a very large number of variations to be interrogated in
parallel
Platforms
- MATRIX PlateMatePlus and Tecan for high throughput liquid
handling
- Illumina Beadstation 500
- Illumina Infinium II and Goldengate assays
- Illumina BeadChip and Array Matrix formats
- Beadstudio data analysis software
Bioinformatics - Analysis Support
Our Bioinformatics scientists provide a complete analysis
package that can also be customised to your specific research
needs using: 
- Illumina BeadStudio data analysis software
- Partek Genomics Suite
- Open source tools
Contact
E-mail: Genotyping
Team