SNP Genotyping Using the Fluidigm® EP1™ System

 

The Technology

 

The high throughput Fluidigm EP1™ System uses the innovative integrated fluidic circuit (IFC). This platform is ideal for mid-multiplex genotyping at very high throughput using IFCs called dynamic arrays™. The matrix of channels, chambers and valves perform the complex work of assembling the arrays.

 

Benefits of IFCs

 

Unlike other liquid handling methods, fluids are pressurised into the chip where reaction volumes are controlled by the internal well geometries. This eliminates the volume variability associated with other procedures. This results in experiments being conducted with less sample and reagent.

 

SNP Genotyping Using Dynamic Array IFCs

 

Why Use Fluidigm IFCs for SNP Genotyping:

 

  • High Data Quality
    ≥99% call rates
    Uses robust gold-standard TaqMan® chemistry
  • High Accuracy
    ≥99.75% accuracy
  • High Throughput
    9,216 data points produced per 96.96 chip
    27,000 data points/genotypes produced per day
  • Fast Workflow
    Results produced in hours
    Short project turnaround time
  • Small Quantities of DNA Required
    Ideal for precious sample analysis

GenotypingEP1pic1

 

Array Types Available

 

GenotypingEP1pic2
Array 48.48 96.96
Number of  Samples/Array 48 96
Number of Samples/Array 48 96
Number of Reactions/Array 2,304 9,216
GenotypingEP1pic3

We also offer the greater flexibility of running larger numbers of sample against smaller numbers of SNP arrays.

 

Bioinformatics - Analysis Support

 

Our experienced bioinformaticians are able to provide a complete analysis package that can be customised to your specific research needs. Please contact us for further details.

 

Why Use Source BioScience LifeSciences™?

 

  • Personalized project design
  • Individual project managers for each project
  • Knowledgeable scientists with considerable PCR experience
  • Specialist bioinformaticians to handle and interpret data
  • Specialist reports supplied
  • Fast turnaround times
  • Comprehensive, competitive quotes

 

Other Services Offered on the Fluidigm EP1 System

 

Fluidigm, EP1, Dynamic Array, Digital Array & NanoFlex are trademarks or registered trademarks of Fluidigm Corporation in the U.S. and/or other countries. All other trademarks are the property of their respective owners. For research use only.