Automated Colony Picking

Automated Colony Picking

High-Throughput & High-Quality Arraying of cDNA or Genomic Clones into Microtiter Plates


Source BioSciences Automated Clone Arraying Service provides fast and easy access to cDNA or genomic libraries arrayed in barcode-labelled 96-well or 384-well microtiter plates. 10,000 to 15,000 clones are routinely arrayed per day.

 

Ordering


For ordering please contact our sales team.

 

Application


Clones arrayed in microtiter plates allow the unambiguous addressing of each clone. Gridded clone collections are the prerequisite for the generation of macroarrays or microarrays, for the generation of ordered clone pools or for PCR amplifications of individual clones.

 

Product Range


Source BioSciences service encompasses the following steps:

 

  • Transformation
  • Titer determination
  • Plating of bacterial suspension onto agar plates
  • Blue/white selection upon request
  • Arraying of clones into barcode-labelled microtiter plates (384-well or 96-well)
  • Generation of mictrotiter plate copies of arrayed libraries
  • Library storage upon request
  • Stringent quality control: >99,9% vital clones, no contaminations with bacteriophages or other non-E. coli microorganisms

 

Product Specifications


Arrayed libraries are certified as "free of contamination". They are delivered on dry ice along with storage and handling recommendations as well as information about the applied barcode.

 

Quality Control

 

Clone arraying and drawing of plate copies applying fully automated devices and sterile working conditions prevents the introduction of cross-contamination and contamination with microorganisms. However, all plates (each clone) is checked by a three step quality procedure. All quality-control results are electronically documented and available to our customers on demand.