Sanger sequencing

Primers and primer storage

Primers currently in stock

 

PRIMER (alphabetically)

SEQUENCE

LENGTH

% G+C

Tm (°C)

30nt_M13R

AGC GGA TAA CAA TTT CAC ACA GGA

24

42

59

ACYC Duet Up1

GGA TCT CGA CGC TCT CCC T

19

63

55

BGH Reverse

TAG AAG GCA CAG TCG AGG

18

56

50

C.elegansRNAi F

GTT TTC CCA GTC ACG ACG TT

20

50

64

CMVF_pCDNA3

CAA CGG GAC TTT CCA AAA TG

20

45

55

Duet Down1

GAT TAT GCG GCC GTG TAC AA

20

50

52

EGFP C F

CAT GGT CCT GCT GGA GTT CGT G

22

59

59

EGFP C R

GTT CAG GGG GAG GTG TG

17

65

62

EGFP Nrev

CGT CGC CGT CCA GCT CGA CCA G

22

73

64

KS

CCT CGA GGT CGA CGG TAT CG

20

65

58

M13R

CAG GAA ACA GCT ATG ACC

18

50

48

M13F

TGT AAA ACG ACG GCC AGT

18

50

48

PBADF

ATG CCA TAG CAT TTT TAT CC

20

35

58

PBADR

GAT TTA ATC TGT ATC AGG

18

33

45

pCMV forward

GAG CTC GTT TAG TGA ACC GTC

21

52

54

pCMV reverse

CAA GGC CAG GAG AGG CAC TG

20

65

58

PDONR201 3PRIME

CAT CAG AGA TTT TGA GAC AC

20

40

54

PDONR201 5PRIME

GCA GTT CCC TAC TCT CGC

18

61

60

PDONR201 Forward

TCG CGT TAA CGC TAG CAT GGA TCT C

25

52

72

PDONR201 Reverse

GTA ACA TCA GAG ATT TTG AGA CAC

24

37

58

pEXPRESS_F

TAT GGC TAG CAT GAC TGG t

19

47

49

PGEX 3'

CCG GGA GCT GCA TGT GTC AGA GG

23

65

62

PGEX 5'

GGG CTG GCA AGC CAC GTT TGG TG

23

65

62

malEforward

GGT CGT CAG ACT GTC GAT GAA GCC

24

58

61

PME18SF

CTT CTG CTC TAA AAG CTG CG

20

50

62

PME18SR

GCA CCT GCA GCT CGA GCA CA

20

65

73

POLYA

AAA AAA AAA AAA AAA AAA AAA AAA B

25

0

57

POLYT

TTT TTT TTT TTT TTT TTT TTT TTT V

25

0

58

PQE30 forward

CCC GAA AAG TGC CAC CTG

18

61

53

PQE30 reverse

GTT CTG AGG TCA TTA CTG G

19

47

49

pTargeT reverse

TTA CGC CAA GTT ATT TAG GTG ACA

24

38

63

pTrcHis_F

GAG GTA TAT ATA TTA ATG TAT CG

23

29

45

SK

CGG CCG CTC TAG AAC TAG TGG ATC

24

58

61

SP6 promoter

ATT TAG GTG ACA CTA TAG

18

33

41

T3

ATT AAC CCT CAC TAA AG

17

35

40

T7 promoter (F)

TAA TAC GAC TCA CTA TAG GG

20

40

48

T7 terminator (R)

GCT AGT TAT TGC TCA GCG G

19

53

51

pCEP-Forward

AGA GCT CGT TTA GTG AAC CG

20

50

52

pCEP-Reverse

GTG GTT TGT CCA AAC TCA TC

20

45

50

pEF1a fwd ATT TGC CCT TTT TGA GTT TGG 21 38 58

 

Disclaimer: Source BioScience accepts no responsibility for failed sequencing reads when customers select any of the above primers but have not checked to see if these primer sequences bind to their template. The above primers do not necessarily have the same content as primers of the same or similar name that can be found elsewhere.

 

Sample Storage

We keep your templates and primers up to 3 weeks, unless instructed otherwise. If you would like any of your materials kept for longer, then simply let us know and we will move them into longer term storage. Materials in long term storage that have not been used for a period of 3 months will be disposed of, unless you instruct us otherwise.