Other sequencing services
Source BioScience LifeSciences offer a range of other DNA
sequencing options so whatever your sequencing needs, we can
help!
DNA cloning
service
We provide
a flexible and efficient cloning service catered to your specific
needs.
We have
vast experience in a variety of cloning projects which we can
combine with any of our other processes, for example:
-
Sequencing
-
Plasmid extraction
-
Clone purchasing
-
PCR optimisation
Please contact
us so we can discuss your requirements, which will help us
ensure that you receive the most relevant information from us.
back to top
Large
construct sequencing service
Full
sequencing of your BAC, YAC or Cosmid clones by Shotgun
Sequencing or
Next Generation Sequencing is also available at Source
BioScience LifeSciences.
Full
sequencing of large constructs such as BAC, YAC or Cosmid
clones/libraries can be challenging and time consuming using Sanger
sequencing. Next Generation Sequencing using the
, Illumina Hi Seq 2000, Illumina MiSeq & Roche 454
GS FLX+ System, has dramatically improved speed and reduced
costs, enabling the full sequencing of large constructs at a
fraction of the costs and time.
Sample
requirements:
Service specifications:
-
Library preparation
-
Cluster generation
-
Sequencing by synthesis
-
Report generation and data delivery
Deliverables:
-
Advanced data analysis available
-
Raw data returned to you in the post via external hard drives
Our
bioinformaticians can help you if you require advanced sequence
analysis (e.g.: de novo assembly).
Please contact
us so we can discuss your requirements, which will help us
ensure that you receive the most relevant information from us.
back to top
GLP-compliant sequencing
Requirements for the project
-
A study plan agreed between the customer and our Study
Director
-
Template DNA, in the format of your choice, is supplied by the
customer
The project itself:
-
A designated Study Director will oversee the comprehensive study
plan
-
Specific primer design will be included within the study, if
required
-
DNA preparation from a bacterial culture or agar plate, if
necessary
-
Quality control of the template DNA
-
Sequencing will be performed on an ABI 3730 XL 96-capillary
sequencer
-
Sequences will be analysed using "ABI base caller" which yields
extremely accurate results
-
Depth of the final data, X-fold coverage, if required
-
All study and data material will be archived
On conclusion of the analysis:
-
A comprehensive report will be produced by our Study Director.
-
Chromatograms primer designs and any other electronic data can be
downloaded from a secure ftp file and/or in CD format.
Please contact
us so we can discuss your requirements, which will help us
ensure that you receive the most relevant information from us.
back to top
Shotgun sequencing
service
Source
BioScience LifeSciences can prepare libraries from samples
including genomes, BACs, fosmids, cosmids and more. All genomic
shotgun libraries are constructed and sequenced within our facility
by our highly experienced scientists.
-
Large DNA fragments are nebulised and cloned into a
standard E. coli vector
-
Only recombinant clones will grow due to the expression of the
lethal gene, ccdB
-
Only circularised, single insert containing vectors will be
propagated
-
T7, T3, M13f and M13r primer sites close to the insert site allows
for more sequencing of the insert and not the vector
Source
BioScience LifeSciences has completed large genomic sequencing
projects on the mouse and pufferfish genomes and we are happy to
provide our expertise to in sequencing and genomic assemblies.
-
Assembly using Staden GAP4
-
Sequencing will be performed on an ABI 3730 XL sequencer
-
Customer will receive the assembly and all reads in either disc, or
ftp file format
Please contact
us so we can discuss your requirements, which will help us
ensure that you receive the most relevant information from us.
back to top