The quality of the template DNA used in automated sequencing is of the highest importance. Templates that are too low in concentration or that contain sequencing reaction inhibitors such as salt, carbohydrate and or protein will all generate sequences low in signal intensity. When signal intensity is low the analysis software has difficulty in resolving the base peaks from background noise. Typically this results in a shorter read length with other random peaks being generated under the main data set.
We recommend that plasmid templates are submitted at 100ng/ul and eluted in pure water.
We offer a Template Amplification Service to enhance the quality and concentration of plasmid templates that have previously proven to be unsuitable for sequencing.
If you would like us to try the Template Amplification on your templates please indicate this on your sequencing order.
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