RNAzol® RT RNA Isolation Reagent

RNAzol® RT separates RNA from other molecules in a single-step, without the use of chloroform.

RNAzol® RT is a quick and convenient reagent for the isolation of high-quality total and small RNA (mRNA and miRNA) from biological samples.

This product also enables sequential isolation of RNA and DNA.

A mixture of guanidine thiocyanate and phenol in a monophase solution, RNAzol® RT dissolves DNA, RNA and protein on homogenisation or lysis of tissue samples.

The addition of water to the RNAzol® RT mixture precipitates DNA, proteins, polysaccharides and other molecules, which are easily removed by centrifugation. RNA is then purified from the supernatant by alcohol precipitation, washing and solubilisation.

RNA isolated using this reagent contains little or no contaminating DNA and protein.

Applications

  • PCR
  • Microarrays
  • Northern blotting
  • RNase protection assays

Advantages

  • Provides a high-yield of good-quality RNA
  • Isolates pure and undegraded RNA that is ready for RT-PCR without DNase treatment
  • Enables easy isolation of mRNA, micro RNA or total RNA
  • Can be used to isolate mRNA and microRNA in separate fractions or to isolate total RNA in one fraction
  • Fast and convenient - RNA can be isolated in less than one hour
  • No chloroform-induced phase separation is necessary
  • Stable at room temperature for both storage and experimental procedure

E01010A
RNAzol®RT RNA Isolation Reagent
50 ml

£44.00
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