miRNA Reagents

The All-in-One™ miRNA qRT-PCR reagent kits include the All-in-One miRNA qRT-PCR Detection Kits and the All-in-One miRNA First-Strand cDNA Synthesis Kits. When used with All-in-One Validated miRNA Primers or miProfile™ miRNA qPCR Arrays, the result is high-performance expression-profiling of all miRNAs.


A novel optimized blend of poly A polymerase and reverse transcriptase with an PAP/RT buffer that was specifically developed for the All-in-One Reverse Transcriptase Mix delivers maximum activity of both enzymes for robust first-strand cDNA synthesis from mature miRNAs. The All-in-One First-Strand cDNA Synthesis Kit is optimized for use with miProfile qPCR Arrays.


The All-in-One RT-PCR Detection Kit includes both RT and PCR reagents. It combines PCR technology and SYBR® Green to make fast and accurate quantification of mature miRNAs from as little as 10 pg of small RNA or 20 pg of total RNA samples. The kit is designed for use with All-in-One Validated miRNA Primers.

All-in-one miRNA assays provides sensitivity and specificity you can afford

  • Detect miRNAs from as little as 10 pg of small RNA or 20 pg of total RNA
  • Detect only mature miRNA
  • Compatible with GeneCopoeia miRNA qPCR arrays or validated miRNA-specific primers

 

All-in-one miRNA assays are fast and easy

 miRNA

Figure 1. Overview of steps involved in the All-in-One miRNA qRT-PCR Detection Kit. During 3' polyadenylation, M-MLV RTase and a unique Oligo dT Adaptor primer, reverse transcribes the poly A miRNAs. The kit qRT-PCR Mix, containing SYBR® Green specifically detects the reverse transcribed miRNAs.

Combine these tools with GeneCopoeia miRNA plasmid constructs and assays for comprehensive functional analysis studies.

  • miExpress™ pre-miRNA expression clones
  • miTarget™ miRNA 3' UTR target clones
  • Luc-Pair™ miR Luciferase Assays

*miRNA-specific primers sold separately.

 

miProfile™ miRNA qPCR Arrays

For high-throughput profiling of miRNA expression

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level. Usually 21-23 nucleotides in length, miRNAs are important modulators in cellular pathways and are highly conserved in eukaryotic organisms. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases.

 

The miProfile miRNA PCR Arrays are designed for profiling the expressions of pre-defined or customized sets of miRNAs in various tissues or cells. The resulting differential expressions of profiled miRNAs help researchers to identify those miRNAs that are of biological significance and importance relevant to their research. Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer) and 12 wells of different types of controls, which are used to monitor the efficiency of the entire experimental process – from reverse transcription to qPCR reaction.

 

Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. A cDNA pool, containing reverse transcript products of 10 different tissue total RNAs, was used as the qPCR validation template.

 

Using a universal real-time PCR condition, one can easily profile and analyze the miRNA expression in a high-throughput fashion.The All-in-One™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended  and supported RT-PCR reagents for use with the miProfile miRNA qPCR arrays. These reagents have been optimized to produce high sensitivity, efficiency, and specificity.

 

Key advantages

  • Validated miRNA primers - Each miRNA primer is designed using a proprietary algorithm and experimentally validated
  • Robust performance
  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
  • Broad linearity– Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible – High reproducibility (R2>0.99) for inter-array and intra-array replicates
  • Genome-wide coverage, pre-arranged groups, or customized groups
  • Largest genome-wide miRNA coverage
  • Cancer-related groups
  • Customised miRNA arrays for focused study

miProfile™ Cancer miRNA qPCR Arrays

For focused group profiling of cancer-related miRNA expression

miProfile cancer miRNA qPCR arrays profile the expression of cancer-related miRNAs, which are carefully chosen for their close cancer correlation based on a thorough literature search of peer-reviewed publications. Arrays are available for expression profiling of specific types of cancer-related miRNAs or overall cancer-related miRNAs.

 

miRNAs are known for their extensive involvement in cancer development and progression and their altered pattern of expression may affect the cell cycle and survival program. The miProfile cancer arrays allow researchers to profile the differential expression of cancer-related miRNAs in normal tissues and various cancers to gain understanding of the role of miRNA in cancer pathogenesis as well as to identify or validate markers for cancer classification, diagnosis, and prognosis.

 

Key advantages

  • Close cancer correlation - miRNAs are carefully chosen for their close cancer correlation based on a thorough literature search of peer-reviewed publications
  • Robust performance
  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
  • Broad linearity– Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible – High reproducibility (R2>0.99) for inter-array and intra-array replicates
  • Validated miRNA primers
  • Each miRNA primer is designed using a proprietary algorithm and experimentally validated

 

miProfile™ Disease and Focus-Group miRNA qPCR Arrays

For focused group profiling of miRNA expression

miProfile disease and focus-group miRNA qPCR arrays profile the expression of disease-related or other focus-group miRNAs, which are carefully chosen for their close disease, pathway, or process correlation based on a thorough literature search of peer-reviewed publications. miRNAs are important modulators in cellular pathways and are highly conserved in eukaryotic organisms. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases. The miProfile disease and focus-group arrays allow researchers to study the differential expression of these miRNAs to gain understanding of the role of miRNA in disease pathogenesis or cellular pathways as well as to identify or validate key markers.

Key advantages

  • Close correlation - miRNAs are carefully chosen for their close correlation to the specific diseases or research focus based on a thorough literature search of peer-reviewed publications
  • Robust performance
  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
  • Broad linearity– Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible – High reproducibility (R2>0.99) for inter-array and intra-array replicates
  • Validated miRNA primers - Each miRNA primer is designed using a proprietary algorithm and experimentally validated

 

miProfile™ miRNome miRNA qPCR Arrays

For high-throughput profiling of miRNA whole genome expression

The miProfile human miRNome miRNA qPCR arrays are a set of nineteen 96-well plates covering 1,565 of best characterized and annotated miRNAs based on miRBase V17. Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer) and 12 wells of different types of controls, which are used to monitor the efficiency of the entire experimental process – from reverse transcription to qPCR reaction.

 

Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. A cDNA pool, containing reverse transcript products of 10 different tissue total RNAs, was used as the qPCR validation template.

 

Using a universal real-time PCR condition, one can easily profile and analyze the miRNA expression in a high-throughput fashion. The All-in-One™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended  and supported RT-PCR reagents for use with the miProfile miRNA qPCR arrays. These reagents have been optimized to produce high sensitivity, efficiency, and specificity.

 

To help best distinguish single-nucleotide mismatched miRNAs, 61 single-nucleotide mismatched miRNAs from the human miRNome are further grouped together on one 96-well plate. The miProfile human single-nucleotide mismatch miRNA qPCR array is available as stand-alone products for users who want to study these miRNAs using specific PCR conditions.

Key advantages

  • Validated miRNA primers - Each miRNA primer is designed using a proprietary algorithm and experimentally validated
  • Robust performance
  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
  • Broad linearity– Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible – High reproducibility (R2>0.99) for inter-array and intra-array replicates
  • Largest coverage
  • More than 1,500 of best characterized and annotated miRNAs are covered based on miRBase V17

miProfile™ custom miRNA qPCR arrays

For expression profiling of custom-specified miRNAs

miProfile™ custom miRNA qPCR Arrays profile the expression of customer specified miRNAs. Each primer is designed using a proprietary algorithm and has been experimentally validated. Depending on the number of miRNAs and replicates to be analyzed, GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate. Different controls can also be included in the arrays to help monitor the sample quality, RT and PCR reaction efficiencies and replicates reproducibility:

  • Spike-in reverse transcription control (RT): monitors the efficiency of the RT reaction.
  • Positive PCR control (PCR): verifies the PCR efficiency.
  • Housekeeping genes (HK): can be used as endogenous positive controls and for array normalization.
  • Negative controls(NC): monitors the sample quality

For the best performance, All-in-One™ miRNA First-Strand cDNA Synthesis Kit and All-in-One™ qPCR Mix are the recommended and supported reagents for use with these arrays.

Key advantages

  • Validated primers - Each primer is designed using a proprietary algorithm and has been experimentally validated
  • Flexible array design - GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate.
  • Robust performance
  • Sensitive – Detect miRNA from as little as 10 pg of small RNA or 20 pg of total RNA
  • Specific – Be able to distinguish miRNAs with single nucleotide mismatches. Each primer has been experimental validated for specific amplification
  • Broad linearity – Allow miRNA at varieties of expression level to be detected simultaneously
  • Reproducible -High reproducibility (R2> 0.99) for inter-array and intra-array replicates