GeneCopoeia All-in-One™ qPCR mix with validated primers provide universal qPCR reaction conditions and robust quantitative PCR data.

The jointly developed and co-optimised All-in-One qPCR mix and gene-specific primers deliver the entire range of advantages you need without any of the high costs you’ve come to expect:

  • Uniform reaction conditions reduce experimental design time enabling earlier journal submissions
  • High amplification efficiency and sensitivity even for low-copy genes means reliable quantitation every time
  • Absence of non-specific amplification* and no primer-dimers* ensures reproducible and ready-to-publish data

The All-in-One qPCR mix uses high-fidelity hot-start polymerase, an optimized reaction buffer and high-quality dNTPs to enable specific and sensitive amplification from even low-copy RNA (cDNA) or DNA species.

All-in-one qPCR validated primers get the job done     

Eliminate endless adjustments and optimizations with precision qPCR from GeneCopoeia. Just add All-in-One primers and start.

The all-in-one qPCR human- mouse- or rat-specific primers are designed by a proprietary algorithm and validated for precision performance. Primer validation includes melting curve to ensure amplification of the correct target DNA (figure 2).

When used in combination with the All-in-One SYBR® Green qPCR Mix, the All-in-One primers deliver reliable and reproducible high performance in quantitative PCR assays.

ExProfile™ gene qPCR arrays

For high-throughput profiling of gene expression

The ExProfile™ gene qPCR arrays are designed for profiling the expressions of pre-made or customised sets of coding-genes in various tissues or cells. The resulting differential expressions of profiled genes help researchers to identify those that are biologically significant and relevant to their research. In each 96-well plate, there are up to 84 pairs of qPCR primers and 12 wells of controls which are used to monitor the efficiency of the entire experimental process – from reverse transcription to qPCR reaction.

Each pair of primers used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted mRNA. A cDNA pool, containing reverse transcript products from total RNA of 10 different tissues, was used as the qPCR validation template.

A universal real-time PCR condition was developed for easy profiling and analysis of  the gene expression in a high-throughput fashion. The All-in-One™ First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended and supported RT-PCR reagents for use with the ExProfile™ gene qPCR arrays. These reagents have been optimized to produce high sensitivity, efficiency, and specificity.

Key advantages

Validated mRNA primers

  • Each primer pair is designed using a proprietary algorithm and has been experimentally validated

Robust performance

  • Sensitive – Detects as low as 4 copies of RNA using ExProfile gene qPCR array and recommended reagents/conditions
  • Broad linearity – Simultaneously detects mRNAs at different expression levels
  • Reproducible – High reproducibility (R2> 0.99) for inter-array and intra-array replicates

Pre-arranged groups, or customised groups

  • Pre-arranged cancer-related groups
  • Pre-arranged pathway-related groups
  • Customized gene arrays for focused study 

ExProfile™ cancer gene qPCR arrays

For focused group profiling of cancer-related gene expression

 

ExProfile™ cancer gene qPCR arrays profile the expression of cancer-related genes, which are carefully chosen for their close cancer correlation based on a thorough literature search of peer-reviewed publications. Arrays are available for expression profiling of specific types of cancer-related genes.

 

In each 96-well catalog array plate there are up to 84 pairs of PCR primers, which have been pre-validated and deposited in designated wells. In each plate there also are 12 wells of controls for monitoring the efficiency of the entire experimental process: from reverse transcription to qPCR reaction.

Key advantages

  • Close cancer correlation - Genes are carefully chosen for their close cancer correlation based on a thorough literature search of peer-reviewed publications
  • Robust performance
  • Sensitive – Detects as low as 4 copies of RNA using ExProfile qPCR array and recommended reagents/conditions
  • Broad linearity – simultaneously detects mRNAs at different expression levels
  • Reproducible -High reproducibility (R2> 0.99) for inter-array and intra-array replicates
  • Validated primers - Each primer is designed using a proprietary algorithm and has been experimentally validated

ExProfile™ custom gene qPCR arrays

For expression profiling of custom-specified genes

ExProfile™ Custom Gene qPCR Arrays profile the expression of customer specified genes. Each primer is designed using a proprietary algorithm and has been experimentally validated. Depending on the number of genes and replicates to be analysed, GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate. Different controls can also be included in the arrays to help monitor the sample quality, RT and PCR reaction efficiencies and replicates reproducibility.

  • Genomic DNA control (GDC): detects genomic DNA contamination.
  • Spike-in reverse transcription control (RT): monitors the efficiency of the RT reaction.
  • Positive PCR control (PCR): verifies the PCR efficiency.
  • Housekeeping genes (HK): can be used as endogenous positive controls and for array normalization.

For the best performance, All-in-One™ First Strand cDNA Synthesis Kit and All-in-One™ qPCR Mix are the recommended and supported reagents for use with these arrays.

Key advantages

  • Validated primers - aach primer is designed using a proprietary algorithm and has been experimentally  validated
  • Flexible array design - GeneCopoeia offers 14 layouts to choose from, including 6 types with 96-well plate and 8 types with 384-well plate.
  • Robust performance
  • Sensitive – Detects as low as 4 copies of RNA using ExProfile™ Gene qPCR Array and recommended reagents/conditions
  • Broad linearity – Simultaneously detects mRNAs at different expression levels
  • Reproducible -High reproducibility (R2> 0.99) for inter-array and intra-array replicate