jetPRIME® is a new powerful and versatile transfection reagent which ensures high DNA transfection efficiency and excellent gene silencing in a variety of adherent cells. jetPRIME® is ideal for DNA/siRNA co-transfection and is very gentle on cells since it requires low amounts of reagent and nucleic acid during transfection. Effective and nontoxic DNA and siRNA delivery is essential for reliable scientific results.

Ordering information

jetPRIME: siRNA, DNA and co-transfection reagent
0.1 ml plus 5 ml buffer

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jetPRIME: siRNA, DNA and co-transfection reagent
0.75 ml plus 60 ml buffer

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jetPRIME, siRNA, DNA and Co-transfection reagent
1.5 ml plus 2 x 60 ml buffer

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jetPRIME, siRNA, DNA and Co-transfection reagent
5 x 1.5 ml plus 10 x 60 ml buffer

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jetPRIME, siRNA, DNA and Co-transfection reagent (with concentrated buffer)
5 x 1.5 ml of jetPRIME + 120 ml of jetPRIME buffer 5 x conc.

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1.5 ml is sufficient to perform ca.375 transfections in 6-well plates. Bulk quantities are available upon request.

Superior DNA transfection efficiency

Superior transfection efficiencies ranging between 70 and 90% were obtained when using jetPRIME® reagent versus the top competitor’s reagent for several commonly used cell lines (Fig. 1).

 Transfection efficiency


Fig. 1. Transfection efficiency assessed by FACS analysis in various cell lines 24h following transfection in 24-well plates according to the manufacturer’s recommendation for competitor L2K  and 0.5 µg plasmid, 1 µl reagent per well for jetPRIME®.



Many other cell lines of various origins, as well as primary cells, are transfected with unusually high percentages (Table 1).

Transfection efficiency table








Table 1. Transfection efficiency of various cell types using jetPRIME®. The percentage of GFP-positive cells was determined by FACS analysis 24 h after transfection. 

Economical: less DNA and less reagent

jetPRIME® is such a powerful in vitro transfection reagent that only requires a small amount of reagent and plasmid DNA (Table 2), making it very economical.

Economy table


Table 2. Amounts of DNA and reagent (jetPRIME® and competitor) added per well in 6-well plate for transfection according to manufacturers' recommendations.


In addition to reducing costs, using less DNA also minimizes adverse cytotoxic effects triggered by transfection. Hence, jetPRIME® is the reagent of choice for high transfection efficiency with excellent cell viability.


Better cell viability

jetPRIME® is extremely gentle on cells during transfection leading to increased cell viability (Fig. 2) and improved transfection results. Cells transfected with jetPRIME® are healthy, while major cytotoxicity is observed with competitor.

Cell viability Cell viability part 2


Fig. 2. Phase contrast microscopy of HeLa cells 24 h after transfections performed according to the manufacturer’s recommendations for each reagent.




Excellent gene silencing

jetPRIME® leads to over 90% knockdown of endogenous gene expression in a variety of cell lines. For example, jetPRIME®-mediated transfection of HeLa cells with 10 nM siRNA duplexes targeting endogenous lamin A/C in HeLa cells drastically reduces lamin A/C gene expression to barely detectable level (Fig. 3).

Gene silencingGene silencing 2


Fig. 3. Endogenous lamin A/C silencing using jetPRIME®. HeLa cells were transfected with 10 nM of 21-mer lamin A/C siRNA.
After 48 h, lamin A/C silencing was assessed by immunofluorescence microscopy using an antibody against lamin A/C.



Cotransfection of DNA and siRNA

jetPRIME® is well suited for DNA and siRNA cotransfection experiments. It shows highly efficient gene silencing in a variety of cell lines with very low toxicity. Over 90% silencing is achieved in adherent cells, using 10 nM siRNA (Fig. 4).




Fig. 4. Exogenous luciferase gene silencing in several cell lines after DNA & siRNA cotransfection using jetPRIME® performed with 400 ng p4CMV-Luc and 10 nM of luciferase siRNA per well in 6-well plates.




shRNA and miRNA plasmid transfection

In mammalian cells, RNA interference can be achieved by other means than synthetic siRNA. Another approach consists in using plasmid-based methods. It has been reported that efficient gene silencing can be obtained with small hairpin RNA (shRNA) plasmids. For transfection of such plasmids, jetPRIME®, our versatile DNA transfection reagent, is recommended.

When studying miRNA (micro-RNA) using a plasmid-based approach, the standard transfection method using jetPRIME® is applicable and ensures reliable transfection efficiency.

Convenient protocol

jetPRIME® is an easy-to-use transfection reagent (Fig. 5):

  • Fast and easy to scale up and down
  • Compatible with serum and antibiotics

Convenient protocol

Fig. 5. jetPRIME™ convenient protocol for DNA, siRNA and co-transfection of DNA and siRNA.