mRNA Transfection

Introducing jetMESSENGER™ for mRNA transfection

Take gene expression to the next level with mRNA transfection and jetMESSENGER!

  • Unmatched transfection efficiency
  • Outstanding results on a wide variety of hard-to-transfect cells, adherent and suspension
  • Outperforms DNA transfection
  • Extremely gentle on cells
  • No risk of plasmid integration
  • Perfectly suited for CRISPR/Cas9 gene editing, iPS generation and stem cell differentiation

Try jetMESSENGER™, request a sample!

jetMESSENGER™ from Polyplus Transfection is a highly efficient and gentle mRNA transfection reagent. mRNA transfection presents many advantages versus DNA transfection:

  •          no risk of genome integration, hence no genome modification of the transfected cell
  •          no promoter regulation issue
  •          no need to reach the nucleus for efficient expression
  •          more gentle process

 jetMESSENGER™ has been specifically designed to offer outstanding transfection efficiency in cells that are usually hard-to-transfect, finally allowing relevant gene expression experiments in almost all cell types.


 Fig1 Efficiency Jetmessenger Vs Competitor

Fig 1. jetMESSENGER™ outperforms its main DNA transfection reagent competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection of EGFP mRNA (5meC, pseudo-uridine, Trilink™) or plasmid DNA encoding for eGFP. Conditions used were according to the manufacturers’ recommendation.


Works on a variety of cell lines

With jetMESSENGER™, achieve outstanding transfection efficiency in a wide variety of cell lines, from adherent and suspension cancer cells, to sensitive primary cells and neurons. jetMESSENGER™ is also perfectly suitable for reprogramming experiments in fibroblasts and stem cells.

Table 1 Cell Efficiency

Table 1. jetMESSENGER™ leads to high transfection efficiency in a wide variety of cell types

Easy to use

mRNA transfection is as easy as DNA transfection: just dilute the mRNA in the provided buffer, add jetMESSENGER™ to the diluted mRNA and add the mix to your cells growth medium in presence/absence of serum (compatible with antibiotics).

Ordering information


Catalog Number

Amount of reagent

Amount of buffer


0.1 ml

10 ml


0.75 ml

60 ml


1.5 ml*

2 x 60 ml

 *1.5 ml is sufficient to perform approx .375 transfections in 6-well plates. Bulk quantities are available upon request. Please contact us.