jetMESSENGER™ from Polyplus Transfection is a highly efficient and gentle mRNA transfection reagent. mRNA transfection presents many advantages versus DNA transfection:
jetMESSENGER™ has been specifically designed to offer outstanding transfection efficiency in cells that are usually hard-to-transfect, finally allowing relevant gene expression experiments in almost all cell types.
Fig 1. jetMESSENGER™ outperforms its main DNA transfection reagent competitor. Transfection efficiency was assessed by FACS analysis in various cell lines 24 h after transfection of EGFP mRNA (5meC, pseudo-uridine, Trilink™) or plasmid DNA encoding for eGFP. Conditions used were according to the manufacturers’ recommendation.
Works on a variety of cell lines
With jetMESSENGER™, achieve outstanding transfection efficiency in a wide variety of cell lines, from adherent and suspension cancer cells, to sensitive primary cells and neurons. jetMESSENGER™ is also perfectly suitable for reprogramming experiments in fibroblasts and stem cells.
Table 1. jetMESSENGER™ leads to high transfection efficiency in a wide variety of cell types
Easy to use
mRNA transfection is as easy as DNA transfection: just dilute the mRNA in the provided buffer, add jetMESSENGER™ to the diluted mRNA and add the mix to your cells growth medium in presence/absence of serum (compatible with antibiotics).
Amount of reagent
Amount of buffer
2 x 60 ml
*1.5 ml is sufficient to perform approx .375 transfections in 6-well plates. Bulk quantities are available upon request. Please contact us.