Purified Inactivated Agents

ABI offers select inactivated agents for research that requires antigens that are no longer infectious. Methods of inactivation include: Detergent Treatment, UV-Inactivation, beta-Propiolactone, and Heat-Inactivation.

Depending on the application, the researcher must be careful in choosing which inactivation method is suitable for their needs.

Detergent: A chemical inactivation method that leaves no intact viral particles. The detergent dissociates most viral proteins. Suitable for ELISA, Western blot, and other protein-based assays.

Ultraviolet: A physical and chemical inactivation method that leaves whole, intact viral particles. UV light crosslinks the agent’s nucleic acid with minimal or no modification of the viral proteins. Suitable for ELISA, Western blot, and other protein-based assays. 

Beta-Propiolactone (ß-PL): A chemical inactivation method that modifies nucleic acid and leaves whole, intact viral particles. ß-PL inactivation causes minimal or no modification of viral proteins. Suitable for ELISA, Western blot, and other protein-based assays.

Heat: A physical inactivation method that leaves whole, intact virus, bacteria, or chlamydia elementary bodies. The heat inactivates the infectious agent, but leaves the nucleic acid unmodified. Suitable for nucleic acid based assays, such as standard and real-time PCR.

 

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