qPCR Controls

ABI’s qPCR Controls from Source BioScience can be used to generate standard curves for quantitative PCR assays and as positive controls in nucleic acid amplification reactions. Quantitated PCR Controls for viruses, bacteria, and other organisms such as Toxoplasma gondii and Mycoplasma pneumoniae are available.

ABI’s Quantitated PCR Controls are purified from one of the following sources:

  • Intact viral particles, parasites or mollicutes
  • Cultured bacteria: pelleted bacteria from liquid, plate, or slant culture
  • Cloned viral DNA

Quantitated PCR Controls are supplied in volumes of 250 µL at 1-2 x 104 DNA copies/µL (except for BK and JC polyomaviruses, and Porcine Circovirus Type 2 (PCV2), which are supplied at 1-2 x 105 DNA copies/µL). Quantitation with different instruments and by different methods may not give the same copy number results.Note: ABI’s Quantitated PCR Controls are intended for use as positive PCR quantitative standards for the organism in question. Due to the nature of these products, ABI cannot guarantee their suitability as extraction controls. Additionally, due to the extreme sensitivity of detection in PCR reactions, and since no method of purification can guarantee the complete absence of extraneous agents, PCR controls are not intended for use as negative controls for other organisms.

ABI’s PCR Controls are for research use only and are not for use in diagnostic assays.

 

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