ATLAS scFV Constructs
To enhance our proteomic resources Source BioScience are now distributing the ATLAS clones constructed and annotated at the Wellcome Trust Sanger Institute by team 86.
Antibodies were selected against 404 antigen targets which represent 280 mouse genes. The antigens were primarily produced in E.coli or within mammalian expression system; the majority of these genes are cell surface receptors.
The scFv constructs are fused to a tri-FLAG tag sequence and the dimeric bacterial enzyme, alkaline phosphatase. This has a dual rationale of driving dimerisation which increases avidity of binding and also acts as a useful enzymatic tag for detection purposes. 22 unique clones were selected for each antigen. Antibodies were characterised by specificity testing and detailed sequence analysis. Furthermore, many of scFvs have been used in immunohistochemistry analysis on tissue microarrays.
Source BioScience offers the complete clone set of 53 96-well microtitre plates or individual clones, for research purposes only. For each antigen, 22 unique scFv constructs are available.
Further details can be found on the reference publication
We can also make subsets of your chosen clones from the collection. Please contact us to discuss your requirements.
Please utilise the AtlasDB antibody database to search for your desired scFv constructs.
Search and Order
The ATLAS scFv clone collection is not currently searchable via our GenomeCUBE® search and cannot be ordered online. We are in the process of integrating the data, however in the meantime you can still order these clones using a purchase order sent by email or fax to our customer services at the contact details below.
To place your order:
1. Search for the clone you require in the databases at the links below and identify the Construct AB_NAME.
AtlasDB antibody database
If you have any problems finding the clone you are looking for please contact us.
2. Use the Construct AB_NAME to place your purchase order.
Price per single clone £87
(Prices exclude VAT and shipping)
For pricing on multiple clones please contact our customer services for a quotation.
3. Print off, read and sign the appropriate Material Transfer Agreement:
4. Send your order and signed Material Transfer Agreement to:
Fax: +44 (0) 115 973 9021