ATLAS scFv constructs Clones

ATLAS scFv constructs clones

 

Please note that the clones being sent have tested negative in our T1 phage contamination assay. However these clones should still be handled with care as no phage assay can be guaranteed to be 100% reliable.

 

THE DISPATCH NOTE SHOWS THE ORIGINAL CLONE ID ORDERED AND ALSO THE ALIAS (PLATE AND WELL NUMBER). THIS IS WHAT IS SHOWN ON THE CLONE LABEL. PLEASE KEEP THE DISPATCH NOTE FOR FUTURE REFERENCE.

 

WHAT TO DO WITH YOUR CLONES WHEN YOU RECEIVE THEM:

 

Plates contain LB broth with 8% glycerol and the appropriate antibiotic. The plates are sent on Dry Ice, and should be stored at -70°C.

 

Individual clones have been streaked onto LB agar containing 30μl/ml Kanamycin. Please store them at 4°C (not in a freezer). As soon as possible, transfer the culture to LB broth containing the antibiotic +8% glycerol and incubate overnight at 30°C (do not try to transfer single colonies, instead scoop out the whole bacterial culture using a disposable inoculation loop). Incubate overnight at 37°C for subsequent freezing at -70°C.

 

These clones are for research purposes only.

 

Acknowledgements and Subsequent Publication:

 

Application of phage display to high throughput antibody generation and characterization.

 

Schofield DJ, Pope AR, Clementel V, Buckell J, Chapple SD, Clarke KF, Conquer JS, Crofts AM, Crowther SR, Dyson MR, Flack G, Griffin GJ, Hooks Y, Howat WJ, Kolb-Kokocinski A, Kunze S, Martin CD, Maslen GL, Mitchell JN, O'Sullivan M, Perera RL, Roake W, Shadbolt SP, Vincent KJ, Warford A, Wilson WE, Xie J, Young JL, McCafferty J.

 

Genome Biol. 2007 Nov 29;8(11):R254 [Epub ahead of print]

 

The Welcome Trust Sanger Institute, Hixton Campus, Cambridge UK