TLR Screening
InvivoGen has developed a high-throughput method to determine
whether a compound is recognized by Toll-Like Receptors and acts
either as an agonist or antagonist. This method is based on
TLR-induced activation of various transcription factors including
NF-κB, AP1 and IRF7 in HEK293 clones.
Key Service Features
• Short turnaround time - Screening turnaround: ONLY 2 weeks
• Screening flexibility - Screening parameters can be selected
and/or modified based on customer requirements. Level 1 and level 2
can be carried out on additional TLRs, including mouse TLRs. More
compound concentrations can be tested.
• Cost effective - A set-up charge applies for the first
compound. Subsequent compounds are heavily discounted.
Description
Two choices of services are offered, level 1 and level 2, that
can be performed sequentially or individually.
Level 1: Single dose testing on a panel of
human TLRs Testing is carried out on seven TLRs.
- TLR2: wide array of microbial molecules
- TLR3: dsRNA
- TLR4: Gram-negative lipopolysaccharide
- TLR5: flagellin
- TLR7: single-stranded RNA and small antiviral molecules
- TLR8: single-stranded RNA and small antiviral molecules
- TLR9: specific unmethylated CpG oligonucleotide sequences
Screening is performed at a single concentration, typically a
1/10 dilution of the original compound solution provided, or
customer specified.
Duplicate tests (plus controls, see list).
Level 2: Dose response on one or two TLRs Three
concentrations of the compound(s), typically 1/10, 1/100 and
1/1000 dilutions of the original compound solution, are tested
on the TLR(s) recognizing the compound(s) as determined in level 1
or specified by the customer. Triplicate tests (plus controls).
TLR ligands are typically recognized by a single TLR,
potentially two (TLR7 and TLR8). Recognition by TLR4 usually
reflects the presence of endotoxins in the sample.
Compounds can be supplied as stock solutions or a solid
materials for water-soluble compounds.
A detailed repor t will be prepared and provided to the customer
electronically and in hard copy. All procedures are performed
accordingly to strict guidelines. Confidentiality is
guaranteed.
NOD Ligand Screening
Two cytoplasmic proteins involved in pathogen recognition, NOD1
and NOD2, can be added to this screening service. Their activation
by a given compound is detected using the same method as the one
described above.TLR Ligands Concentration
TLR2 HKLM 108 cells/ml
TLR3 Poly(I:C) 25 μg/ml
TLR4 E.coli K12 LPS 100 ng/ml
TLR5 S. typhimurium flagellin 1 μg/ml
TLR7 Imiquimod 3 μg/ml
TLR8 ssPolyU/LyoVec 10 μg/ml
TLR9 CpG ODN 2006 10 μg/ml
NOD1 Tri-DAP 1 μg/ml
NOD2 Muramyl dipeptide 100 ng/ml
TLR / NOD Ligand Screening tlrl-test contact us for further information